AP Biology 2023 Mrs. Jones’ Responses # 1
The addition of a phosphate group to a protein could cause the protein to have a conformational
shape change. Due to this shape change, the protein can go from an active to an inactive form.
During the process of a phosphorylation cascade, the intermediates that are activated will
phosphorylate additional components in the pathway which will also activate additional
components. For example, the activated receptor protein will activate one protein kinase A, this
protein kinase A will activate 10 intermediate proteins, then those 10 intermediate proteins will
activate 10 each of their target intermediate. As you see this signal is being amplified.
The dependent variable is the variable that is measured due to the independent variable. The
researchers changed the type of mutation (WT, pho81mt, pho4mt) in the yeast strains, which is
the independent variable. The researchers measured the activity of the APase enzyme and the
amount of mRNA for PHO1 that encodes the APase enzyme, which are the dependent variables.
The researchers used the wild-type (WT) strain as a negative control. This allows the researchers
to establish a baseline of the levels of APase and amount of PHO1 mRNA in each environment
(without the treatment) to compare with the treatment groups to determine the effect of Pho81
and Pho4 on the activation of the target genes.
ADDED: The wild-type has all of the genes for the PHO genes. By using the wild-type, you are able to
ensure the gene with the mutation is the one being tested.
By using a specific mutant, the researchers are able to isolate each protein on the overall pathway
to see the specific function of each. Pho81 inhibits Pho80/Pho85 so the Pho4 is not
phosphorylated, so if this process was inhibited by a mutant Pho81 in low-phosphate environment.
Pho4 interacts with the DNA as a transcription factor, so if the Pho4 was non-functional.